1. Principle
This test kit is based on the competitive enzyme immunoassay. The enzyme conjugate is pre-coated on the micro-well stripes. The residue enrofloxacin in the testing sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibody against enrofloxacin. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of residue enrofloxacin in it. This value is compared to the standard curve and the content of the corresponding enrofloxacin residue is subsequently obtained.